Bio Rad Thermal Cycler Gradient Pcr

I m trying to set up a gradient pcr but the machine won t allow me to choose the temperatures in the gradient beyond the front and back row low. This pcr machine comes with a gradient enabled 384 well fast reaction module for high throughput and can transition easily to a real time pcr by swapping the block for a cfx optical module. Using a temperature gradient allows you to evaluate 8 or 12 annealing polymerization or denaturation temperatures in a single run depending on the cycler model.

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